Tissue specific metabolite and lipid analysis using MALDI-FT-MS – linking spatial distribution to metabolic function

Tissue specific metabolite and lipid analysis using MALDI-FT-MS – linking spatial distribution to metabolic function

Tissue specific metabolite and lipid analysis using MALDI-FT-MS – linking spatial distribution to metabolic function (#016)

Ute Roessner ¹ ² , Berin A Boughton ¹ , Daniel L Sarabia ²

Metabolomics Australia, School of BioSciences, The University of Melbourne, Melbourne, VIC, Australia
School of BioSciences, The University of Melbourne, Melbourne, VIC, Australia

Imaging Mass Spectrometry (IMS) is a novel technique allowing the measurement of the spatiotemporal distribution of many biomolecules, including lipids and metabolites, in thin sections of biological tissues. The growing field of spatial lipidomics and metabolomics is developing and adopting IMS as a complementary analytical tool to conventional ‘omics analyses of crude tissue extracts. Here we present the advantages and disadvantages of Ultrahigh Mass Resolution Matrix Assisted Laser Desorption Ionisation Fourier Transform Ion Cyclotron Resonance IMS and describe potential applications of spatial lipid and metabolite analysis in different biological tissues such as from mammals or plants. We will discuss how IMS will contribute to our increasing knowledge base of biological systems under investigation by providing information on the spatial distribution of lipids and metabolites.

Authors contributing to this presentation.

Roessner, U

Prof Roessner has obtained her Diploma in Biochemistry at the University of Potsdam and the John Innes Institute in Norwich, UK after which she pursued a PhD in Plant Biochemistry at the Max-Planck-Institute for Molecular Plant Physiology in Germany, where she developed novel GC-MS methods to analyse metabolites in plants. Together with the application of sophisticated data mining, the field of metabolomics was born and is today an important tool in biological sciences, systems biology and biomarker discovery. In 2003 she moved to Australia where she established a GC-MS and LC-MS based metabolomics platform as part of the Australian Centre for Plant Functional Genomics for which she led the node at the University of Melbourne. In addition, since 2007, Prof Roessner has been involved in the setup and lead of Metabolomics Australia (MA), a federal and state government funded national metabolomics service facility and now leads the MA node at the University of Melbourne. In 2013, Prof Roessner was awarded an Australian Research Council Future Fellowship to establish her own research program applying Imaging Mass Spectrometry for spatial metabolite and lipid analyses to understand root metabolism under salinity stress. Currently her research program uses metabolomics and lipidomics technologies to decipher how plant roots interact with beneficial microbes under abiotic stress conditions. In 2018, Prof Roessner took up the position as Head of School, School of BioSciences, University of Melbourne.

Tissue specific metabolite and lipid analysis using MALDI-FT-MS – linking spatial distribution to metabolic function (#016)

Roessner, U

Boughton, B.A

Sarabia, D.L

Tissue specific metabolite and lipid analysis using MALDI-FT-MS – linking spatial distribution to metabolic function (#016)

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Roessner, U

Boughton, B.A

Sarabia, D.L

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