The advent of high resolution Q-Orbitrap MS-instruments allowed for quantification of peptides/proteins with high sensitivity and selectivity using PRM targeted analysis. Time-scheduled PRM acquisition allows reducing the duty cycle time hence increasing the number of peptides that can be targeted. However unavoidable drifts in peptide elution time can lead to missing targets and often chromatographic windows of 2-3 minutes have to be used while actual elution times last around 0.5 min. This results in inefficient use of instrument time (below 25%). Here we present a novel data acquisition scheme that allows for significantly increasing the number of peptides targets in a PRM assay.