PROTEOMICS 2016* Vendor Workshops
FRIDAY 5th FEBRUARY 2016
Thermo Fisher Scientific Breakfast Workshop
Time: 7:30 - 9:00am Location: Heritage Ballroom, Mantra Lorne
Confirmed Speakers
Prof. Matthias Mann, Max Planck Institute of Biochemistry, Germany
“Technology development towards high-throughput proteomics”
Prof Andrea Sinz, Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, D-06120 Halle (Saale), Germany
“An Integrated Workflow for Structural Proteomics Studies based on Cross-linking/Mass Spectrometry with an Orbitrap Fusion Mass Spectrometer”
Cross-linking combined with MS has evolved as an alternative strategy in structural biology for characterizing three-dimensional structures of protein assemblies and for mapping protein-protein interactions. One of the most pressing questions to be addressed in the chemical cross-linking/MS approach is the development of automated workflows. In my talk, I will describe an integrated workflow for an automated identification of cross-linked products that is based on the use of an MS/MS cleavable cross-linker generating characteristic reporter ion patterns upon fragmentation. I will evaluate different fragmentation methods available on an Orbitrap Fusion mass spectrometer (CID, HCD, ETciD, and EThcD) in combination with a dedicated software tool, MeroX, for conducting fully automated analyses of cross-linked products. The confidence of cross-link assignment is validated by a decoy search strategy. Moreover, I will show ETD data of intact cross-linked protein/peptide complexes.
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Bruker Afternoon Workshop: Gain Deeper Biological Insight by Accelerating Your Omics Research
Time: 2:45pm - 4:15pm | Afternoon drinks and snacks will be served between 4:15 pm – 4:45pm Location: Heritage Ballroom, Mantra Lorne
Confirmed Speakers
Prof. Matthias Mann, Max Planck Institute of Biochemistry, Germany
"In depth proteomics with the impact II and beyond”
Prof. Peter Hoffmann, Adelaide Proteomics Centre, University of Adelaide
“MALDI TOF Mass Spectrometry Imaging on Cancer Tissue and MALDI TOF Mass Spectrometry Profiling on Beer”
Dr. Andrew Webb, Walter+Eliza Hall Institute of Medical Research
“Dissecting disease biomarkers using intact protein profiling with UHR-QTOF MS”
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Agilent Technologies Afternoon Workshop: Technologies and Tools to Assist Disruptive and Orthogonal Approaches for Proteomics
Time: 2:45pm - 4:15pm | Afternoon drinks and snacks will be served between 4:15 pm – 4:45pm Location: Heritage 2, Mantra Lorne - Refreshments provided
Confirmed Speakers
Dr. David Bradley & Tom Hennessy, Agilent Technologies, Australia
Dr. Hyun Joo An, ChungNam National University, South Korea
Christine Miller, Agilent Technologies, Santa Clara, United States
Program
2:45-2:50pm Welcome & Introduction & Review Agenda
Dr David Bradley
2:55-3:15pm Agilent Technologies Academia Team & Program
Dr David Bradley & Tom Hennessy
3:15-3:45pm Decoding Glycosyl Modification of Biotherapeutics by Intact Protein Analysis using Mass
Spectrometry
Dr Hyun Joo AN
3:45-4:15pm Technologies and Tools to Assist Disruptive and Orthogonal Approaches for Proteomics
Christine Miller
Agilent Technologies’ Academia Team & Programs
What you will learn:
- Meet the Academia & Collaborations Team
- Unique programs specifically designed to facilitate academic research & collaborations
- Mutual benefits by design: reflection on programs’ focus, science, connections, relevance & outcomes
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SATURDAY 6th FEBRUARY 2016
SCIEX Breakfast Workshop: Next-Generation Sequencing meets Next-Generation Proteomics
Time: 7:30am - 9:00am Location: Heritage Ballroom, Mantra Lorne
Confirmed Speakers
Dr. Christie Hunter - SCIEX, United States
Dr. James Broadbent - SCIEX
As a first step in enabling such research on a large scale, SCIEX has partnered with Illumina to enable processing of SWATH® next-generation proteomics data on BaseSpace®, Illumina’s cloud computing environment. OMICs processing in the cloud promises; up to 10x faster processing of SWATH® data, a user friendly app environment for integrating new tools, easier collaboration and sharing with colleagues worldwide and the ability to perform data management and analysis from any web-equipped computer.
In this workshop, SCIEX Field Application Specialist James Broadbent will demonstrate the latest versions of the Protein Extractor and Workflow Manager applications that enable the conversion of raw SWATH data files to detailed protein expression data and visuals in fast, intuitive manner. The Workflow Manager also incorporates the Protein Expression Analytics application, which provides the mass spectrometrist a detailed review of the data quality, such as data completeness, precision and false discovery rates.
Following the software demonstration, SCIEX’s Global Director of OMICs Applications, Christie Hunter, will outline the first multi-omics study to be conducted using BaseSpace®. In this study - a collaboration between SCIEX, Advaita and researchers from the University of California, San Francisco (UCSF) - cytotrophoblast (CTB) cells were studied to investigate their role in placental abnormalities. In this investigation, transcriptomics and SWATH™ proteomics data from CTB cell extracts were processed in BaseSpace and the results combined in Advaita’s iPathway guide, providing a seamless processing pipeline from data to meaningful biological answers.
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Novachem Breakfast Workshop: The inside story on Peptiquant™ and how it will change your research forever
Time: 7:30 - 9:00am Location: Heritage 2, Mantra Lorne
Confirmed Speaker
Dr. Gary Kruppa - MRM Proteomics Inc, Canada
Researchers in academia and life science industries continue to adopt a targeted, bottom-up MS-based proteomic workflow for biomarker discovery and validation. Biomarker validation requires absolute quantification of surrogate peptides in the sample matrix, a requirement that is best achieved using stable isotope enriched peptide internal standards (SIS). Although the use of SIS has increased the reliability of LC/MRM-MS based assays, optimal results require properly functioning equipment and a workflow with minimal human error and bias.
To help researchers establish a stable LC/MRM-MS platform for bottom-up quantitative proteomics, MRM Proteomics in conjunction with Cambridge Isotopes Limited now offer PeptiQuant™ products. These innovative products are used to assess and track LC/MS performance of the LC/MS platform in a proteomic workflow in order to highlight any possible issues affecting quantitation.
In addition to doing away with the less accurate and more expensive ELISA and Western Blot protein quantitation methods, PeptiQuant™ now provides researchers with a variety of customisable biomarker assessment assays which provide a huge advantage in biomarker identification for specific disease profiles and allow results to be accurately verified with high levels of certainty.
The workshop with Gary Kruppa, CEO of MRM Proteomics promises a wealth of insight into how the technology works, some real-life examples of the benefits of the methods and an opportunity to ask your questions about how PeptiQuant™ can benefit your research.
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